荷斯坦奶牛乳腺干细胞的成神经诱导鉴定及催乳素对其增殖活性的影响

doi:10.11843/j.issn.0366-6964.2018.03.010

Abstract

Abstract:

This study investigated the multi-directional differentiation potential of mammary stem cells in Holstein cows and the effect of prolactin on its proliferative activity. The Nissl staining was performed to identify the nerve cells induced from the mammary stem cells in Holstein cows, and the marker genes of neuronal cells were analyzed by RT-PCR. The effect of prolactin on the proliferation of mammary stem cells was detected by MTT.The results indicated that the neuron-like cells and microtubule-like structure were found during induction of mammary stem cells, which was positive by Nissl staining. The marker gene (β-Tubulin Ⅲ and NSE) of nerve cells were positive expression after induction. Prolactin could improve the proliferation of mammary stem cells in Holstein cows, and the optimal proliferation concentration of prolactin to mammary stem cells was at 100 ng·mL^-1.

CLC Number:

S823.9⁺1.2

LIU Ying-chun, CHEN Yu-meng, HAN Zhe-xian, GAO Feng, ZHOU Huan-min. Induction and Identification of Neural Cell from Mammary Stem Cells in Holstein Cows and the Effect of Prolactin on Its Proliferative Activity[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 542-548.

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Induction and Identification of Neural Cell from Mammary Stem Cells in Holstein Cows and the Effect of Prolactin on Its Proliferative Activity

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